mouse anti microsomal triglyceride transfer protein large subunit mtp  (Santa Cruz Biotechnology)

Journal: Clinical and Experimental Medicine

Article Title: The impact of magnesium biotinate and arginine silicate complexes on metabolic dysfunctions, antioxidant activity, inflammation, and neuromodulation in high-fat diet-fed rats

doi: 10.1007/s10238-024-01434-9

Figure Lengend Snippet: Effects of magnesium biotinate (MgB) and inositol-stabilized arginine silicate (ASI) on liver Niemann-Pick C1-Like 1 (NPC1L1, a ), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR, b ), cholesterol 7-alpha-monooxygenase (CYP7A1, c ), acetyl-CoA acetyltransferase 2 (ACAT2, d ), and microsomal triglyceride transfer protein (MTP, e ) levels in high-fat diet (HFD)-fed rats ( n = 8). Data show the relative intensity of the western blot bands expressed as a percent of the control set at 100%. β-actin normalization was used to ensure equal protein loading. Representative blots are shown in each panel. The error bars above the lines point out the standard deviation of the mean. Different symbols (a–d) indicate statistical differences among the groups ( P < 0.05; ANOVA and Tukey post hoc test)

Article Snippet: These membranes were then blocked with a 5% solution of bovine serum albumin at room temperature for two hours before overnight incubation at 4 °C with antibodies targeting IL-1β, IL-6, TNF-α, IRS-1, PPAR-γ, SREBP-1c, ATP citrate lyase (ACLY), FAS, Niemann-Pick C1-Like 1 (NPC1L1), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), cholesterol 7-alpha-monooxygenase (CYP7A1), acetyl-CoA acetyltransferase 2 (ACAT2), and microsomal triglyceride transfer protein (MTP, Santa Cruz Biotechnology, CA, USA).

Techniques: Western Blot, Control, Standard Deviation